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shrna against p53  (Addgene inc)


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    Structured Review

    Addgene inc shrna against p53
    Shrna Against P53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+against+p53/pCE-mp53DD+(Plasmid+%2341856)/pm41081995-72-18-21
    Average 95 stars, based on 81 article reviews
    shrna against p53 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    shRNA:

    Article Title: Combining chromosome conformation capture and exome sequencing for simultaneous detection of structural and single-nucleotide variants
    Article Snippet: .. Reprogramming of human peripheral blood mononuclear cells (PBMC) into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41,813), MYC and LIN28 (addgene #41,855), shRNA against p53 (addgene #41,856), SOX2 and KLF4 (addgene #41,814), EBNA1 (addgene # 41,857), according to the previously described method [ ]. .. Briefly, 5 × 10 5 PBMC were transfected with 1 μg of each vector using the Neon Transfection System (Thermo Fisher Scientific) under optimized conditions (1650 V, 10 ms, 3 pulses).

    Article Title: Combining chromosome conformation capture and exome sequencing for simultaneous detection of structural and single-nucleotide variants.
    Article Snippet: .. Exome enrichment was performed using KAPA HyperExome Probes and KAPA HyperCap kit according to the manufacturer’s instructions. iPS cells generation and differentiation Reprogramming of human peripheral blood mononuclear cells (PBMC) into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41,813), MYC and LIN28 (addgene #41,855), shRNA against p53 (addgene #41,856), SOX2 and KLF4 (addgene #41,814), EBNA1 (addgene # 41,857), according to the previously described method [14]. .. Briefly, 5 × 105 PBMC were transfected with 1 μg of each vector using the Neon Transfection System (Thermo Fisher Scientific) under optimized conditions (1650 V, 10 ms, 3 pulses).

    Article Title: DNA hypomethylation at specific CG-sites within TRAK1 is linked to the neurocognitive profile in Klinefelter syndrome.
    Article Snippet: KS amniotic fluid-derived cell lines X4, X5, and X6 were obtained from the Coriell Institute, Camden, NJ, USA, under the identifiers GM02269, GM03091, and GM03535, respectively, and karyotypically normal control male amniocytes M7, M8, and M9 were obtained from Dr. Daniel L. Van Dyke at the Mayo Clinic, Rochester, MN, USA. .. Briefly, 1×106 amniocytes from each line were reprogrammed via nucleofection with three non-integrating plasmids overexpressing human SOX2 and KL4 (pCXLE-hSK, plasmid #27078, Addgene, Watertown, MA, USA), human L-MYC and LIN28 (pCXLE-hUL, plasmid #27080, Addgene, Watertown, MA, USA), and human OCT3/4 and shRNA against p53 (pCXLEOCT3/4-shp53, plasmid #27077, Addgene, Watertown, MA, USA) and plated onto gelatinized plates in DMEM media containing 10% FBS and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA). .. Following six-seven days in culture, cells were re-plated at single-cell density onto mouse embryo fibroblast (MEF) feeders in F12 media consisting of DMEM/F12, 20% Knockout Serum Replacer, 1x non-essential amino acids, 1x Glutamax100, β-ME, and 1% penicillin-streptomycin (all from Gibco, Grand Island, NY, USA), supplemented with the HDAC inhibitors SAHA and Sodium Butyrate (Sigma, St. Louis, MO, USA).

    Article Title: Development of a Stable Human iPSC Line from Peripheral Blood: A Control Resource for Disease Modeling
    Article Snippet: PBMCs at a density of 5 × 10 6 cells/ml were cultured in SFEM II medium (STEMCELL technologies, 09605) supplemented with growth factors SCF (50 ng/ml), IGF (40 μg/ml), EPO (3 U/ml), and IL-3 (10 μg/ml). .. On day 8, PBMCs were nucleofected with three episomal plasmids (pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, and pCXLE-hUL) encoding pluripotency factors OCT4 with shRNA against p53, SOX2, KLF4, L-MYC, and LIN28 (Addgene, #27077, #27078, #27080), in a 1:1:2 ratios. .. Nucleofection was performed with 0.5 million cells using the Invitrogen Neon Transfection System under the following conditions: 1300 V, 30 ms pulse width, and 1 pulse.

    Article Title: POLR3A mutations cause nucleolus abnormalities and aberrant telomerase RNA metabolism in induced pluripotent stem cells from Wiedemann-Rautenstrauch premature aging syndrome patient.
    Article Snippet: Comparing the progeroid phenotype in cell models of distinct premature aging syndromes is critical for identifying early and common aging hallmarks.. In this study, using a non-integrative episomal approach we reprogrammed iPSCs from cells of a patient suffering from Wiedemann-Rautenstrauch Syndrome (WRS), which is caused by bi-allelic pathogenic mutations of the RNA polymerase III subunit A gene (POLR3A).. In parallel, an iPSC line with the classic HGPS caused by a lamin A mutation was obtained.

    Article Title: Method of making induced pluripotent stem cells using p53 inhibitors
    Article Snippet: .. Alternatively, as an expression vector that encodes an shRNA against p53, a viral vector such as retrovirus, prepared on the basis of a commercially available plasmid (for example, pMKO.1-puro p53 shRNA2: #10672, commercially available from Addgene, and the like) or the like can also be used. ..

    Article Title: DNA hypomethylation at specific CG-sites within TRAK1 is linked to the neurocognitive profile in Klinefelter syndrome
    Article Snippet: KS amniotic fluid-derived cell lines X4, X5, and X6 were obtained from the Coriell Institute, Camden, NJ, USA, under the identifiers GM02269, GM03091, and GM03535, respectively, and karyotypically normal control male amniocytes M7, M8, and M9 were obtained from Dr. Daniel L. Van Dyke at the Mayo Clinic, Rochester, MN, USA. .. Briefly, 1×10 6 amniocytes from each line were reprogrammed via nucleofection with three non-integrating plasmids overexpressing human SOX2 and KL4 (pCXLE-hSK, plasmid #27078, Addgene, Watertown, MA, USA), human L-MYC and LIN28 (pCXLE-hUL, plasmid #27080, Addgene, Watertown, MA, USA), and human OCT3/4 and shRNA against p53 (pCXLE-OCT3/4-shp53, plasmid #27077, Addgene, Watertown, MA, USA) and plated onto gelatinized plates in DMEM media containing 10% FBS and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA). .. Following six-seven days in culture, cells were re-plated at single-cell density onto mouse embryo fibroblast (MEF) feeders in F12 media consisting of DMEM/F12, 20% Knockout Serum Replacer, 1x non-essential amino acids, 1x Glutamax-100, β-ME, and 1% penicillin-streptomycin (all from Gibco, Grand Island, NY, USA), supplemented with the HDAC inhibitors SAHA and Sodium Butyrate (Sigma, St. Louis, MO, USA).

    Plasmid Preparation:

    Article Title: DNA hypomethylation at specific CG-sites within TRAK1 is linked to the neurocognitive profile in Klinefelter syndrome.
    Article Snippet: KS amniotic fluid-derived cell lines X4, X5, and X6 were obtained from the Coriell Institute, Camden, NJ, USA, under the identifiers GM02269, GM03091, and GM03535, respectively, and karyotypically normal control male amniocytes M7, M8, and M9 were obtained from Dr. Daniel L. Van Dyke at the Mayo Clinic, Rochester, MN, USA. .. Briefly, 1×106 amniocytes from each line were reprogrammed via nucleofection with three non-integrating plasmids overexpressing human SOX2 and KL4 (pCXLE-hSK, plasmid #27078, Addgene, Watertown, MA, USA), human L-MYC and LIN28 (pCXLE-hUL, plasmid #27080, Addgene, Watertown, MA, USA), and human OCT3/4 and shRNA against p53 (pCXLEOCT3/4-shp53, plasmid #27077, Addgene, Watertown, MA, USA) and plated onto gelatinized plates in DMEM media containing 10% FBS and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA). .. Following six-seven days in culture, cells were re-plated at single-cell density onto mouse embryo fibroblast (MEF) feeders in F12 media consisting of DMEM/F12, 20% Knockout Serum Replacer, 1x non-essential amino acids, 1x Glutamax100, β-ME, and 1% penicillin-streptomycin (all from Gibco, Grand Island, NY, USA), supplemented with the HDAC inhibitors SAHA and Sodium Butyrate (Sigma, St. Louis, MO, USA).

    Article Title: Method of making induced pluripotent stem cells using p53 inhibitors
    Article Snippet: .. Alternatively, as an expression vector that encodes an shRNA against p53, a viral vector such as retrovirus, prepared on the basis of a commercially available plasmid (for example, pMKO.1-puro p53 shRNA2: #10672, commercially available from Addgene, and the like) or the like can also be used. ..

    Article Title: DNA hypomethylation at specific CG-sites within TRAK1 is linked to the neurocognitive profile in Klinefelter syndrome
    Article Snippet: KS amniotic fluid-derived cell lines X4, X5, and X6 were obtained from the Coriell Institute, Camden, NJ, USA, under the identifiers GM02269, GM03091, and GM03535, respectively, and karyotypically normal control male amniocytes M7, M8, and M9 were obtained from Dr. Daniel L. Van Dyke at the Mayo Clinic, Rochester, MN, USA. .. Briefly, 1×10 6 amniocytes from each line were reprogrammed via nucleofection with three non-integrating plasmids overexpressing human SOX2 and KL4 (pCXLE-hSK, plasmid #27078, Addgene, Watertown, MA, USA), human L-MYC and LIN28 (pCXLE-hUL, plasmid #27080, Addgene, Watertown, MA, USA), and human OCT3/4 and shRNA against p53 (pCXLE-OCT3/4-shp53, plasmid #27077, Addgene, Watertown, MA, USA) and plated onto gelatinized plates in DMEM media containing 10% FBS and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA). .. Following six-seven days in culture, cells were re-plated at single-cell density onto mouse embryo fibroblast (MEF) feeders in F12 media consisting of DMEM/F12, 20% Knockout Serum Replacer, 1x non-essential amino acids, 1x Glutamax-100, β-ME, and 1% penicillin-streptomycin (all from Gibco, Grand Island, NY, USA), supplemented with the HDAC inhibitors SAHA and Sodium Butyrate (Sigma, St. Louis, MO, USA).

    Expressing:

    Article Title: Method of making induced pluripotent stem cells using p53 inhibitors
    Article Snippet: .. Alternatively, as an expression vector that encodes an shRNA against p53, a viral vector such as retrovirus, prepared on the basis of a commercially available plasmid (for example, pMKO.1-puro p53 shRNA2: #10672, commercially available from Addgene, and the like) or the like can also be used. ..



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